1150 Alturas Drive, Suite 105, Moscow, ID 83843
(208) 882-9736
biotracking@biotracking.com

Blood Sampling & Forms

Download Poster

  1. Use a fresh bleeding needle with every cow. Cross-contamination of blood will affect the results.
  2. Screw the Vacutainer needle onto the needle holder.
  3. Insert the Vacutainer tube into the other end of the needle holder until the stopper touches the back portion of the needle. Do not puncture the stopper. Hold these assembled materials in one hand.
  4. Lift the cow’s tail with the other hand.
  5. Insert the needle about 1/2 inch deep and perpendicular to the tail at the underside, midline and at about 3 to 6 inches from the base of the tail. In this region, there is a longitudinal, midline ridge of skin through which you push the needle.
  6. Blood will appear at the junction of the stopper and the back portion of the needle once the vein is punctured. If it does not appear, pull the needle out slightly and insert in a different direction until the vein is punctured.
  7. Once blood is seen, push the tube onto the needle. Be sure to keep the needle under the skin since vacuum will pull blood into the tube and if lost, blood cannot be collected.
  8. Use a second tube if this happens.
  9. Collect 2 cc or more of blood.
  10. Withdraw the needle from the skin and remove the tube from the tube holder.
  11. Sequentially label the sample vials with the ear tag ID using a permanent marker on the vial LABEL. Labeling the tube will assist the laboratory in sample organization and help expedite results.
  12. Place the tube in an ice rack or refrigerator until shipment.
  13. Unscrew the Vacutainer needle from the needle holder and discard the needle into a “sharps” container and re-use the needle holder.
  14. Completely fill out the BioPRYN Pregnancy Test Form. Please be certain that the total number of samples sent is correct.
  15. Return samples to the laboratory in a box. Shipping Instructions
  16. Utilize FedEX, USPS or UPS to ship samples to the laboratory. Make certain that the BioPRYN Pregnancy Test Submission Form accompanies the samples.
  17. Payment for services may be enclosed in packaging.
  18. Don’t forget to recheck pregnant cows tested at 28 days again between 70 and 80 days as a certain percentage will experience early embryonic death loss. Confirmation pregnancy checks are very important for this reason.

Questions, Information, and Supply Requests – contact BioTracking Inc. or one of the BioPRYN affiliate laboratories

Phone: (208) 882-9736

E-mail: biotracking@biotracking.com

  1. Clip the animal’s neck to see the jugular vein. Use electric shears to shave a patch approximately 4 inches wide by 8 inches long. Shaving an area allows for easier viewing of the vein and provides a clean area in order to minimize the chance of introducing dirt or bacteria into the vein with the needle.
    Note: Blood sampling can be done with assistance or alone; however, producers who are new at blood sampling will benefit from having assistance.
  2. The assistant should turn the head of the animal at a 30-degree angle to the side by holding the animal under its jaw to allow for easy access to the vein. The animal’s body may also need to be restrained.
  3. Another assistant or a blocking stand can help keep the animal from
    moving. Restraining an animal without assistance is better for those who have become proficient at drawing blood. The handler should straddle the animal, place his or her knees behind the animas’s shoulders, and back the animal into a corner or against a wall to help control her hindquarters.
  4. The animas’s head should be turned opposite to the side of collection, once again at a 30-degree angle. Restraint of the head is accomplished by using the elbow and the upper arm to keep it held off to the side. This leaves both hands available for the blood collection. An unruly animal can be dangerous to the assistants, the person drawing the blood, and to itself. It is important to be gentle and patient when restraining the animal.
  5. The easiest way to locate the vein is to draw an imaginary line from the middle of the animal’s eye down the side of her neck. The vein can be located by applying pressure with the thumb or fingers in the groove on either side of the trachea and below the half-way point of the shaved area. The pressure will cause the vein to pop up and be easy to see.
  6. Once the vein has been located, the area needs to be properly cleaned to keep bacteria out of the needle insertion site. This is accomplished using surgical scrub on the area.
  7. Apply a small amount of the surgical scrub to a few pieces of gauze. Squeeze some of the excess scrub out of the gauze before applying it to the animal to make the process easier.
  8. The area should be cleaned by starting in the center and working out toward the edge. Never go back over a place that has already been wiped, which may allow bacteria to be carried back into the disinfected area.
  9. Once the area has been cleaned and the vein has been located, the blood can be drawn. This can be done using a needle, needle holder and a blood collection tube.
  10. The needle holder should be guided into place with the right hand while the left hand is used to apply pressure to the vein. The vein should be easy to see and feel. Try to aim for the center so you will have more of a chance of placing the needle within the vein.
  11. Inserting the needle does not require great force. Apply just enough pressure to break through the skin and enter into the jugular vein.
  12. Once the needle is in place, apply pressure so that the blood collection tube is pushed onto the needle. If the needle is in the vein, blood will start to fill the container immediately. If this does not happen, gently withdraw the needle so that the tip comes to the outside of the wall of the vein and re-insert. Gentle prodding may be needed to achieve maximum blood flow.
  13. Collect 2 cc or more of blood.
  14. Before removing the needle, the handler should be sure to remove their left hand, to prevent blood from exiting through the insertion site. Also, be sure to remove the blood collection tube from the needle holder to prevent the loss of the vacuum in the blood collection tube. If the needle is removed from the skin first, the vacuum will be lost and a new blood collection tube will be needed.
  15. After the needle has been removed from the skin, press fingertip over the area where the needle was inserted. A small red dot may appear on the animal’s neck from the needle insertion site. This is normal and is nothing about which to be concerned.
  16. Label the sample vials sequentially with the ear tag or ID. Use a permanent marker on the vial LABEL. Labeling the tube correctly will assist the laboratory in sample organization and help speed results.
  17. Place the tube in the refrigerator until shipment.
  18. Unscrew the needle from the needle holder, discard the needle into a “sharps” container and re-use the needle holder. A new needle must be used for each animal.
  19. Completely fill out the BioPRYN Testing Submission Form. Please be certain that the total number of samples sent is correct. Return samples to the laboratory in a box. Shipping Instructions
  20. Utilize FedEX, USPS or UPS to ship samples to the laboratory. Make certain that the BioPRYN Pregnancy Test Submission Form accompanies the samples. Payment for services may be enclosed in packaging.
  21. The testing laboratory will notify the client of test results via fax, phone or email.

Questions, Information, and Supply Requests – contact BioTracking Inc. or one of the BioPRYN affiliate laboratories

Phone: (208) 882-9736

E-mail: biotracking@biotracking.com

Please contact Herd Health Diagnostics for Wildlife and Equine Testing