Q. What is BioPRYN®wild?

A. BioPRYNwild is a blood based pregnancy test for ruminants and has specific appeal for the U.S. Wildlife industry because it delivers fast, accurate, safe and economical pregnancy diagnostic results. The technology works on all ruminants, including cattle, sheep, goats, bison, deer, elk and moose.

Q. How does BioPRYNwild detect pregnancies?

A. BioPRYN evaluates the blood (more specifically, the serum or plasma) of ruminants for a protein called Pregnancy Specific Protein B (PSPB). PSPB is produced by the placenta, and therefore pregnant animals will have the protein in their blood. This makes the test more accurate than earlier attempts at pregnancy diagnosis that evaluated blood or milk for progesterone or other hormones that can occur in normally cycling animals.

BioPRYNwild uses enzyme-linked immunosorbent assay (ELISA) technology for processing, which contributes to its low cost and fast turn-around.

Q. How early in a pregnancy can animals be tested with BioPRYNwild?

A. Wildlife species (deer, elk, moose, caribou & African antelope) can be tested at 40 days post breeding or greater. Care must be taken to assure that 40 days have passed since breeding or animals may not test accurately. In artificial insemination situations, timing is known. If males are removed from the female for 40 days, this is a good time to test.

Q. What is the accuracy of BioPRYNwild?

A. BioPRYN is greater than 99 percent accurate when the test identifies animals, at least 40 days post breeding, as not pregnant, with only 1 percent showing false-open (false-negative). Correct open detection is very important because giving prostaglandin to misdiagnosed pregnant cows will cause abortion.

The false-pregnant (false-positive) rate for the test is approximately 5 percent. It is presumed that a portion of this variance is due to higher early embryonic death, and not to test inaccuracy.

Inaccuracies can also occur because of mislabeling of samples or cross contamination of one sample with another. A new needle and tube must be used for each animal.

Q. How much does BioPRYNwild?

A. The price of the test is $20 for laboratory processing and there are additional costs for blood collection supplies.

Q. What are the advantages of using BioPRYNwild, versus other methods of pregnancy diagnosis?

A. BioPRYNwild provides wildlife managers early pregnancy detection using an accurate and convenient method while keeping the embryo safe.

Rectal palpation can be highly reliable at various stages of pregnancy, but can cause undue stress for the animal based on the size of the rectal opening of the animal and size of the hand and arm of the technician performing the palpation. BioPRYNwild provides early detection and is non-invasive and embryo safe, reducing the risk of damaging a pregnancy and causing embryo loss

Ultrasound relies on the accuracy of a veterinarian and coordination of both veterinarian and manager’s schedules. Blood sampling for BioPRYNwild can be conducted by the manager, at their convenience. This cost effective and preferred means of pregnancy detection in wildlife delivers accurate results just 40 days post-breeding.

Q. How long does it take to receive the test results?

A. Click here for BioTracking's most up-to-date testing schedule. Reports are sent via phone, fax, mail, or e-mail, depending upon the preference of the recipient.

Q. How should blood samples be prepared and shipped?

A. The veterinarian or manager should collect a minimum of 2 ccs of whole blood (or ship 1 cc of serum) from each animal. Label each tube at minimum with a tube sequence number (e.g. 1,2,3, etc.) and additionally with the animal's ID. It is important to draw samples using individual, disposable needles, to avoid cross-contamination between animals. Needles and tubes can be obtained from veterinarians or veterinarian supply companies.

Tubes containing blood samples should not be opened and should be packed in a well-padded box to avoid breakage. They do NOT need to be packaged in ice. Samples may stay in transit for several days (two-weeks or more) without compromising the results of the test.

Shipping instructions

Q. Who manufactures BioPRYNwild?

A. Dr. R. Garth Sasser of BioTracking Inc. developed the BioPRYNwild test while a researcher and faculty member at the University of Idaho, from where he later licensed the technology. BioTracking Inc. is the sole manufacturer and distributor of BioPRYNwild.

BioTracking Inc. and several affiliate laboratories located across the United States conduct the BioPRYN test for sheep, goats, beef and dairy cattle. However, the BioPRYNwild test is only conducted at the BioTracking laboratory in Moscow, Idaho.

Q. How can a producer get started using BioPRYNwild?

A. Producers should contact BioTracking at (208) 882-9736. BioTracking will provide detailed shipping instructions and establish a system for returning results to the producer.

Q. To whom is the test available?

A. Thanks to the availability of efficient shipping methods, samples can processed from researchers, wildlife managers and veterinarians throughout the United States as well as Canada and Mexico. Veterinarians who serve deer and elk managers also may wish to regularly pool samples from several herds and ship them for processing.

Q. Where can I find more information about BioPRYNwild?

A. To learn more about BioPRYNwild, contact BioTracking Inc.

Phone: (208) 882-9736

E-mail: biotracking@biotracking.com