Q. What is BioPRYNes?

A. BioPRYNes is an ELISA test that measures the amount of estrone sulfate in the serum or plasma of horses as well as other animals. BioPRYN may be used to indicate pregnancy in horses 70 days post breeding up until 2 weeks prior to foaling.

Q. How does BioPRYNes detect pregnancies?

A. Concentration of estrone sulfate is low in serum of non-pregnant mares or in mares in the early stages of pregnancy. Estrone sulfate gradually increases to a distinctly higher level in the mare’s plasma by day 70 of gestation. From this time forward, the concentration continues to increase and remain significantly different than that found in the non-pregnant mare until the last two weeks of pregnancy. Due to this high concentration in plasma or serum, it is possible to measure estrone sulfate as evidence of pregnancy in the mare.

Q. How early in a pregnancy can mares be tested with BioPRYNes?

A. Bred mares can be tested for pregnancy using BioPRYNes any time after 70 days of gestation and up until two weeks before foaling.

Q. What is the accuracy of BioPRYNes?

A. The ELISA test is a measure of the amount of estrone sulfate in the serum or plasma of horses as well as other animals and was developed by Stabenfeldt et al., J. Reprod. Fertil., Suppl. 44 (1991) 37-44. This same assay is used at BioTracking Inc. as the BioPRYNes test. The test is very sensitive and can measure from 1.5 picograms to 1000 picograms per test-well while using 20 microliters of plasma. Thus, this will measure serum or plasma from the mare containing 0.075 to 50 nanograms per cc. The ELISA was accurate in that it reliably measured near 100% of the estrone sulfate, in varying known quantities, that was added to gelding serum. The assay is precise in that repeated measures within an assay had 8.2, 13.2 and 15.1% coefficients of variation (% CV) for high, medium and low amounts of estrone sulfate in the sample. Between assays, the % CV’s were 10.1, 15.3 and 18.9% for high, medium and low amounts of estrone sulfate in the sample. The mean, plus or minus the standard error of the mean, for estrone sulfate in serum of pregnant mares at 70 days after breeding was 9.5 to 12.6 nanograms per cc of serum or plasma. At Day 70 after AI, 95% of pregnant mares will have estrone sulfate concentration in this range. See Stabenfeldt et al. for more on proof of assay.

Q. How much does BioPRYNes cost?

A. The cost for laboratory analysis is $25.00 per sample. Additional costs for testing would include blood collection tubes, needles and shipping.

Q. What are the advantages of using BioPRYNes?

A. BioPRYNes blood sampling is simple to do and can be done by the owner or veterinarian within their schedule. The blood based test may also be less expensive than other testing procedures.

Q. When is the test performed?

A. Click here for the most up to date testing schedule at BioTracking Inc. Reports are sent via phone, fax, mail, or e-mail, depending on the method indicated on the sample submission form.

Q. Who manufactures BioPRYNes?

A. BioPRYNes is assembled and formatted at BioTracking Inc. located in Moscow, Idaho.

Q. How can a horse owner get started using BioPRYNeses?

A. To get started, horse owners can ship 2 ccs of whole blood or 1 cc of serum along with a sample submission form to BioTracking Inc. For detailed shipping instructions, see the side bar to the left and for up-to-date pricing and schedule information, please click here. You may also contact BioTracking Inc. at (208) 882-9736 for more information.

Q. To whom is the test available?

A. Thanks to the availability of efficient shipping methods, BioTracking Inc. can process samples from equine owners throughout the United States. Veterinarians who serve horse breeders and owners also may wish to regularly pool samples from several farms and ship them for processing.

Q. Where can I find more information about BioPRYNes ?

A. To learn more about BioPRYNes, contact BioTracking Inc.

Phone: (208) 882-9736

E-mail: biotracking@biotracking.com