Q. What is BioPRYN®?

A. BioPRYN is a blood pregnancy test for ruminants, and has specific appeal for the U.S. dairy and meat goat industries because it delivers fast, accurate, safe and economical pregnancy detection results. The technology works on all ruminants, including cattle, sheep, goats, bison, deer, elk and moose.

Q. How does BioPRYN detect pregnancies?

A. BioPRYN evaluates the blood (more specifically, the serum or plasma) of ruminants for a protein called Pregnancy Specific Protein B (PSPB). The PSPB is produced by the placenta, and therefore pregnant animals will have the protein in their blood. This makes the test more accurate than earlier attempts at pregnancy diagnosis which evaluated blood or milk for progesterone or other hormones that can occur in normally cycling animals.

BioPRYN uses enzyme-linked immunosorbent assay (ELISA) technology for processing, which contributes to its low cost and fast turn-around.

Q. How early in a pregnancy can animals be tested with BioPRYN?

A. Goats can be tested at 30 days or later, after breeding. Animals that are detected open can then be immediately returned to aggressive breeding programs by natural means of by using hormones.

Q. What is the accuracy of BioPRYN?

A. BioPRYN is greater than 99 percent accurate when the test identifies animals at least 30 days post-breeding as not pregnant with less than 1 percent showing false-nonpregnant (false-negative). Correct detection of non pregnant animals is very important because giving prostaglandin to misdiagnosed pregnant doe will cause abortion.

The false-pregnant (false-positive) rate for the test is approximately 5 percent. A portion of this variance is due to higher early embryonic death, and not to test inaccuracy.

Inaccuracies can occur because of mislabeling of samples or cross contamination of one sample with another. A new needle and tube must be used for each animal.

Q. How much does BioPRYN cost?

A. The test itself costs $6.50 from the laboratory that processes it (this price varies by laboratory), plus the cost of a sample tube and needle. Shipping expenses also must be added if the tests are not processed locally.

Q. What are the advantages of using BioPRYN versus other methods of pregnancy diagnosis?

A. Producers incur less cost using BioPRYN compared to the cost of ultrasound. BioPRYN allows producers to diagnose pregnancy very early with no risk of damaging the fetus. Animals detected open can then be re-bred more quickly, resulting in more re-breedings during the breeding season, more young born per year, and higher lifetime production from each doe.

The test is convenient because on-farm personnel can draw and ship blood samples, rather than working around schedules. This also frees up the veterinarian to concentrate on more complex medical problems with the farm’s management team.
No test is more accurate than BioPRYN. Thus, fewer animals are misdiagnosed and this can amount to a considerable savings in establishing pregnancy and by reducing days open.

Q. How long does it take to receive the test results?

A. The test requires 24 hours from laboratory set-up to reporting. Testing schedules vary by laboratory, please contact your local lab prior to sending samples.

Q. How should blood samples be prepared and shipped?

A. Farm personnel draw blood samples from the jugular vein, which is an easy-to-learn procedure. How to Use Blood samples of at least 2 mL per animal should be collected in individual vacuum tubes and labeled with each animal’s identification number. It is important to draw samples using individual, disposable needles, to avoid cross-contamination between animals, and to assure testing accuracy. Needles and tubes can be obtained from BioTracking, veterinarians or veterinarian supply companies.

Tubes containing blood samples should not be opened and should be packed in a well-padded box to avoid breakage. They do NOT need to be packaged in ice. Samples may stay in transit for several days without compromising the results of the test. Fastest results, however, are achieved when samples are shipped via Federal Express, UPS, U.S. postal service, or other overnight carrier methods. Less expensive second or third day promise of delivery is reliable for most carriers.

Shipping instructions

Q. Who manufactures BioPRYN?

A. Dr. R. Garth Sasser of BioTracking Inc. developed the BioPRYN test using technology licensed from the University of Idaho. BioTracking Inc. is the sole manufacturer of BioPRYN.

Q. How can a producer get started using BioPRYN?

A. Producers should contact BioTracking or one of the BioPRYN affiliate laboratories for detailed shipping instructions and answer any questions that the producer may have about the process. Be sure to check with the laboratory for their testing schedule and pricing.

Q. To whom is the test available?

A. Thanks to the availability of efficient shipping methods, BioTracking Inc. or one of the BioPRYN affiliate laboratories can process samples from farms throughout the United States and internationally. Veterinarians or others who serve smaller farms also may wish to group samples from several farms and ship them for processing.

Q. Where can I find more information about BioPRYN?

A. To learn more about BioPRYN, contact BioTracking Inc. or one of the BioPRYN affiliate laboratories

Phone: (208) 882-9736

E-mail: biotracking@biotracking.com