Q. What is CAEV/OPPV?

A. CAEV (Caprine Arthritis Encephalitis Virus) and OPPV (Ovine Progressive Pneumonia Virus) are persistent lentivirus infections of goats and sheep. Molecular analysis indicates that CAE virus and OPP virus are very similar and they are often grouped together under the name small ruminant lentivirus (SRLV).

Q. How does the VMRD, Inc. cELISA test kit detect CAEV and OPPV?

A. VMRD’s competitive enzyme-linked immunosorbent assay (cELISA) is licensed to detect antibodies to caprine arthritis-encephalitis virus (CAEV) in goat sera and antibodies to ovine progressive pneumonia virus (OPPV) in sheep sera.

Q. How often should a producer test for CAEV or OPPV?

A. The SRLV test is a "snapshot in time" and the CAE/OPP virus can lay dormant for months or years before it begins to reproduce and become infectious. Therefore routine testing is important if you want to maintain your herd as CAE or OPP negative. We recommend consulting with your veterinarian for testing frequency recommendations.

Q. When should goats or sheep be tested for CAEV or OPPV?

A. A marginal result can be an indication of a recent CAE/OPP exposure as antibodies are building up in the serum, or this may be the result of a false positive, possibly from the presence of non-CAE/OPP antibodies which can interfere with the test. We suggest you wait for 6 – 8 weeks after any vaccinations, injuries or illness symptoms are gone and resubmit for a confirmation.

Kids less than 6 - 9 months old may test positive for CAE/OPP if they have received colostrum or milk containing CAE/OPP antibodies. We suggest retesting these kids after nine months of age to verify their status.

Q. What is the accuracy of the SRLV ELISA test?

A. Field research has shown that the SRLV test has a sensitivity of 100% and specificity of 99.6% for caprine and a sensitivity of 95% and a specificity of 98% for ovine.

Q. What are the symptoms for CAEV and OPPV?

A. Polyarthritis is the main clinical sign of CAEV infection, while OPP is typically manifest with labored breathing and emaciation caused by progressive pneumonitis. Most SRLV-infected sheep and goats show no clinical disease but remain persistent carriers of the virus. For more information regarding symptoms of CAE/OPP we recommend consulting with your veterinarian.

Q. How are CAEV and OPPV transmitted?

A. The major mode of viral transmission is vertically through milk and colostrum. Respiratory secretions and feces also harbor infectious virus.

Q. How much does the SRLV ELISA test cost?

A. Please visit BioTracking’s webpage for up-to-date pricing information. http://www.biotracking.com/?q=lab.

Q. How can I prevent the spread of CAEV and OPPV in my herd?

A. Good management practices, supported by a reliable diagnostic tool are the best means of controlling the spread of disease. We recommend consulting with your veterinarian for preventative care and management of the virus.

Q. How long does it take to receive the test results?

A. Please visit BioTracking’s webpage to obtain up-to-date schedule information. http://www.biotracking.com/?q=lab.

Q. How do I ship samples for SRLV ELISA testing?

A. Please visit BioTracking’s webpage to obtain detailed shipping information. http://www.biotracking.com/goats/CAE/shipping.

Q. What type of blood collection tube do I need and how much sample is required?

A. We recommend collecting 2 ccs of blood in a plain red top or red and gray serum separator tube. Tubes should be labeled at minimum with a tube sequence number and additionally with an animal ID or registration number.

Q. How do I create an account for testing?

A. Download the SRLV sample submission form from BioTracking’s webpage (http://www.biotracking.com/?q=lab) fill it out completely and include the form inside the package with the samples. BioTracking will create an account for you based upon the information submitted on the form.

Q. Where can I obtain more information about CAEV or OPPV?

A. To obtain more information about CAE or OPP, we recommend consulting with your veterinarian or contacting the USDA Animal and Plant Health Inspection Service office or website. http://www.aphis.usda.gov/wps/portal/aphis/home.