How to use

How To Use

Gathering serum BVD sample

1. Use a fresh bleeding needle with every cow. Cross-contamination of blood will affect the results.

2. Screw the Vacutainer needle onto the needle holder.

3. Insert the Vacutainer tube into the other end of the needle holder until the stopper touches the back portion of the needle. Do not puncture the stopper. Hold these assembled materials in one hand.

4. Lift the cow’s tail with the other hand.

5. Insert the needle about 1/2 inch deep and perpendicular to the tail at the underside, midline and at about 3 to 6 inches from the base of the tail. In this region, there is a longitudinal, midline ridge of skin through which you push the needle.

6. Blood will appear at the junction of the stopper and the back portion of the needle once the vein is punctured. If it does not appear, pull the needle out slightly and insert in a different direction until the vein is punctured.

7. Once blood is seen, push the tube onto the needle. Be sure to keep the needle under the skin since vacuum will pull blood into the tube and if lost, blood cannot be collected.

8. Use a second tube if this happens.

9. Collect 2 cc or more of blood.

10. Withdraw the needle from the skin and remove the tube from the tube holder.

11. Label the sample vials sequentially and with the animal ID using a permanent marker on the vial LABEL. Labeling the tube will assist the laboratory in sample organization and help expedite results.

12. Place the tube in a refrigerator until shipment.

13. Unscrew the Vacutainer needle from the needle holder and discard the needle into a “sharps” container and re-use the needle holder.

14. Completely fill out the BioTracking BVD Submission Form. Please be certain that the total number of samples sent is correct.

15. Ship the samples to the laboratory in a box. (Link to “Procedure for shipping” instructions)

16. Utilize FedEX, USPS or UPS to ship samples to the laboratory. Make certain that the BioTracking BioPRYN® BVD Sample Submission Form accompanies the samples.

17. Payment by check or credit card for services may be enclosed in package, otherwise an invoice will be sent via email or mail once the samples are received.

Questions, Information, and Supply Requests – call 866-876-9736

Gathering ear notch BVD sample

1. Label the sample vials sequentially and with the animal ID using a permanent marker on the vial LABEL. Labeling the tube will assist the laboratory in sample organization and help expedite results.

2. Prepare 3 containers for use in cleaning the notching tool:
a. Fill 2 containers with clean tap water
b. Fill 1 contain with diluted Nolvasan disinfectant solution (1oz or 30ml Nolvasan diluted in 1 gallon of water).

3. Using the BioTracking approved ear notching tool, take a full notch sample from the tip of the ear, on the top edge.

4. Place the ear notch in the sample vial and replace the cap. Check the numbering of the vials to frequently to make sure the correct animal’s identification number is on the corresponding labeled vial.

5. Keep the notching tool as clean as possible to minimize contamination by first rinsing the tool in the container of water, then in the Nolvasan disinfectant solution, then the third container of water.

6. Once all samples are collected, keep refrigerated until shipment.

7. Completely fill out the BioTracking BVD Submission Form. Please
be certain that the total number of samples sent is correct.

8. Ship the samples to the laboratory in a box. (See "Shipping Instructions")

9. Utilize FedEX, USPS or UPS to ship samples to the laboratory. Make certain that the BioTracking BioPRYN Pregnancy Test Submission Form accompanies the samples.

Questions, Information, and Supply Requests – call 866-876-9736